A MECHANISM FOR THE DETOXIFICATION OF DIMETHYL PHOSPHATE TRIESTERS By D. H. HUTSON, B. A. PICKERING and C. DONNINGER
نویسندگان
چکیده
1. 2-Chloro-1-(2,4,5-trichlorophenyl)vinyl dimethyl phosphate (tetrachlorvinphos) is demethylated by mammalian liver supernatant (100000g) protein in the presence of GSH. 2. GSH acts as an acceptor of the transferred methyl group to form S-methyl glutathione. 3. The enzyme that catalyses this reaction is present in the soluble fraction of liver from mouse, rat, rabbit and pig at similar activity. The enzyme was purified 45-fold from pig liver, dimethyl 1-naphthyl phosphate being used as assay substrate. 4. Methyl groups are readily removed from most of the substrates studied; ethyl groups are removed at one-fiftieth to one-hundredth of the rate for methyl groups. It is likely that the enzyme plays an important role in the detoxification of the phosphate triester pesticides containing CH3-O-P groups.
منابع مشابه
The oxidative dealkylation of insecticidal phosphoric acid triesters by mammalian liver enzymes.
1. The dealkylation of the insecticidal phosphoric acid triester, 2-chloro-1-(2,4-dichlorophenyl)vinyl diethyl phosphate, proceeds in mammalian liver slices via an oxidative mechanism and not by hydrolysis. 2. The enzyme that catalyses the reaction is located in the microsomal fraction of liver homogenate and is dependent for activity on molecular oxygen and NADPH. 3. There are large species di...
متن کاملAcyl-CoA esters of xenobiotic carboxylic acids as biochemically active intermediates.
Crayford, J . V. & Hutson, D. H. (1980) Xenobiorica 10, 349-354 Fears, R . & Richards, D. H. (1981) Biochem. SOC. Trans. 9 , 5 7 2 Fears, R., Baggaley, K . H.. Alexander, R., Morgan, B. & Hindley, R. M. (1978) J. Lipid Res. 19, 3-1 1 Fears, R., Baggaley, K. H., Walker, P . & Hindley, R. M. (1982) Xenobiotica 12,427 -433 Hutson, D.H. (1982) in Progress in Pesticide Biochemistry (Hutson, D. H. & ...
متن کاملThe Detoxification of Methamidophos as an Organophophorus Insecticide on the Magnetite (Fe3O4) Nanoparticles/Ag-NaY Faujasite Molecular Sieve Zeolite (FMSZ) Composite
This work undertakes the study of the detoxification reactions of O, S-dimethyl phosphoramidithioate (methamidophos) as an organophophorus insecticide on the 14.7 wt% magnetite (Fe3O4) nanoparticles/Ag-NaY faujasite molecular sieve zeolite (FMSZ) composite. The influence of solvent (methanol, acetonitrile and n-hexane) on the detoxification potential of composite was inves...
متن کاملThe Detoxification of Methamidophos as an Organophophorus Insecticide on the Magnetite (Fe3O4) Nanoparticles/Ag-NaY Faujasite Molecular Sieve Zeolite (FMSZ) Composite
This work undertakes the study of the detoxification reactions of O, S-dimethyl phosphoramidithioate (methamidophos) as an organophophorus insecticide on the 14.7 wt% magnetite (Fe3O4) nanoparticles/Ag-NaY faujasite molecular sieve zeolite (FMSZ) composite. The influence of solvent (methanol, acetonitrile and n-hexane) on the detoxification potential of composite was inves...
متن کاملOne-dimensional Uranium(VI) Coordination Polymer Complex Containing Dimethyl and Trimethyl Phosphate Ligands: Synthesis, Spectroscopic Characterization, Thermal Analyses, and Crystal Structure
A new one-dimensional uranium(VI) coordination polymer, [UO2(μ-DMP)2(TMP)]n (1) (DMP is dimethyl phosphate and TMP is trimethyl phosphate), was prepared from the reaction of UO2(NO3)2.6H2O and TMP in in THF (THF is tetrahydrofuran) as a solvent. Suitable crystals of this complex for crystal structure determination were obtained by slow evaporation of the produced yellow solution at room tempera...
متن کامل